Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions

The mangabeira (Hancornia speciosa Gomes) is a fruit tree of high potential that distributed in several regions the Brazil, but which great social economic importance for Northeast region. Due to wide possibility using its fruits can be consumed natura or industrialized, and represent financial source traditional communities practice extractivism this species. objective study was evaluate efficiency droplet vitrification technique long-term conservation five accessions, as well verify if there any different behavior among accessions possible effects cryopreservation process. apical shoot tips were subjected periods (30 50 min test I; 25, 75 II) exposure plant solution 2 (PVS2) before plunging into liquid nitrogen. To cryoprotective solutions exposed times on not nitrogen, percentage regeneration observed at 30th 60th day vitro cultivation. It cryopreserve from studied through technique. Adjusts must made steps pre-culture post-culture media so mitigation damage caused during cryopreservation.